Product Details
Catalog Number: MO22180
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Dilutions: Western Blot: 1:5,000-1:10,000. Immunocytochemistry/Immunofluorescence and Immunohistochemistry: 1:1,000-1:5,000.
Immunogen: Pig brain tubulin
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Primate
Entrez: 203068
UniProt: P07437
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ug$325.00Add to Cart

Tubulins are the major protein components of cytoplasmic microtubules. β-tubulin is regarded as a “house keeping” protein which is generally not altered much in expression as a result of experimental manipulations. As a result antibodies to β-tubulin are widely used as loading controls in western blotting experiments as a standard by which the levels of other proteins may be measured. The antibody is an ideal western blotting standard as it stains a single ~50kDa band on western blots and also produces beautiful IF images of cells in tissue culture. It will also work well in ICC and IHC. Mouse select image at left for larger view.

Images
Beta-Tubulin Immunofluorescence

Immunofluorescence analysis of HeLa cells stained with mouse mAb to β-tubulin, MO22180, dilution 1:5,000 in green, and costained with chicken pAb to lamin A/C, dilution 1:2,000, in red.

Beta-Tubulin Immunofluorescence

Immunofluorescence analysis of HeLa cells stained with mouse mAb to β-tubulin, MO22180, dilution 1:5,000 in green, and costained with chicken pAb to lamin A/C, dilution 1:2,000, in red.

Beta-Tubulin Western Blot

Western blot analysis of equal amounts of cell line and whole brain lysates using mouse mAb to β-tubulin, MO22180, dilution 1:5,000 in green.

Beta-Tubulin Western Blot

Western blot analysis of equal amounts of cell line and whole brain lysates using mouse mAb to β-tubulin, MO22180, dilution 1:5,000 in green.