Images
Immunofluorescent

Immunofluorescent analysis of transfected HEK293 cells with a GFP-construct in green stained with mouse antibody to GFP, MO22184, dilution 1:1,000 in red. The blue is Hoechst staining of nuclear DNA. The MO22184 antibody reveals GFP protein expressed only in transfected cells, as a result transfected cells are appeared express both red and green signals and so appear an orange-golden color. Untransfected cells show neither signal.

Immunofluorescent

Immunofluorescent analysis of transfected HEK293 cells with a GFP-construct in green stained with mouse antibody to GFP, MO22184, dilution 1:1,000 in red. The blue is Hoechst staining of nuclear DNA. The MO22184 antibody reveals GFP protein expressed only in transfected cells, as a result transfected cells are appeared express both red and green signals and so appear an orange-golden color. Untransfected cells show neither signal.

Western Blot

Western blot analysis of HEK293 cell lysates using mouse mAb to GFP, MO22184, in green, dilution 1:1,000: [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct, and [4] cells transfected with mCherry construct. The strong green band at ~27kDa corresponds to GFP protein detected only in cells transfected with GFP construct, the antibody does not recognize mCherry.

Western Blot

Western blot analysis of HEK293 cell lysates using mouse mAb to GFP, MO22184, in green, dilution 1:1,000: [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct, and [4] cells transfected with mCherry construct. The strong green band at ~27kDa corresponds to GFP protein detected only in cells transfected with GFP construct, the antibody does not recognize mCherry.

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