Product Details
Catalog Number: MO22184
Applications: ICC, WB, IHC, IF
Type: Mouse IgM
Dilutions: IF/ICC: 1:1,000-5,000 Immunohistochemistry: 1:1,000-5,000 Western Blot: 1:1,000-5,000
Immunogen: Recombinant Aequoria coerulescens GFP purified from E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: liquid
Species Reactivity: Not Applicable
Entrez: 7011691
UniProt: P42212
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$303.00Add to Cart

GFP (green fluorescent protein) is 27kDa protein; which was original isolated from the jellyfish (Aequoria victoria). This antibody was made against a recombinant GFP, from the Aequoria species, referred to as AcGFP. This antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals.

Images
Immunofluorescent

Immunofluorescent analysis of transfected HEK293 cells with a GFP-construct in green stained with mouse antibody to GFP, MO22184, dilution 1:1,000 in red. The blue is Hoechst staining of nuclear DNA. The MO22184 antibody reveals GFP protein expressed only in transfected cells, as a result transfected cells are appeared express both red and green signals and so appear an orange-golden color. Untransfected cells show neither signal.

Immunofluorescent

Immunofluorescent analysis of transfected HEK293 cells with a GFP-construct in green stained with mouse antibody to GFP, MO22184, dilution 1:1,000 in red. The blue is Hoechst staining of nuclear DNA. The MO22184 antibody reveals GFP protein expressed only in transfected cells, as a result transfected cells are appeared express both red and green signals and so appear an orange-golden color. Untransfected cells show neither signal.

Western Blot

Western blot analysis of HEK293 cell lysates using mouse mAb to GFP, MO22184, in green, dilution 1:1,000: [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct, and [4] cells transfected with mCherry construct. The strong green band at ~27kDa corresponds to GFP protein detected only in cells transfected with GFP construct, the antibody does not recognize mCherry.

Western Blot

Western blot analysis of HEK293 cell lysates using mouse mAb to GFP, MO22184, in green, dilution 1:1,000: [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct, and [4] cells transfected with mCherry construct. The strong green band at ~27kDa corresponds to GFP protein detected only in cells transfected with GFP construct, the antibody does not recognize mCherry.