Product Details
Catalog Number: MO22185
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Dilutions: IF/ICC: 1:1,000 IHC: 1:1,000 WB: 1:1,000-2,000
Immunogen: C-terminal 398 amino acids of human ankyrin 3 expressed in and purified in E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: liquid
Species Reactivity: Human, Rat
Entrez: 288
UniProt: Q12955
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 uL of 1 mg/ml$275.00Add to Cart

Ankyrin 3, also known as Ankyrin G, is larger than the related Ankyrin 1 and Ankyrin 2.  Ankyrin 3 is expressed in the axon initial segment and the nodes of Ranvier in the nervous system. Antibodies against Ankyrin 3 thus can be useful in identifying the regions of axon initial segments and nodes of Ranvier. This antibody was made against the C-terminal 398 amino acid of human isotype 1, and works well on human and rat ankyrin 3 on western blots and section material. However, this antibody does not work on mouse extracts and tissues.

Images
Immunofluorescent

Immunofluorescent analysis of cortex section of rat brain stained with mouse antibody against ankyrin 3 (MO22185), dilution 1:2,000 in green and costained with rabbit antibody against NF-L (RA22104) dilution 1:5,000 in red.

Immunofluorescent

Immunofluorescent analysis of cortex section of rat brain stained with mouse antibody against ankyrin 3 (MO22185), dilution 1:2,000 in green and costained with rabbit antibody against NF-L (RA22104) dilution 1:5,000 in red.

Western Blot

Western blot analysis of different tissue and cell lysates using mouse mAb to ankyrin 3, MO22185, dilution 1:2,000 in green: [1] protein standard (red), [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, [6] SH-SY5Y cells, [7] rat brain, and [8] mouse brain lysates.

Western Blot

Western blot analysis of different tissue and cell lysates using mouse mAb to ankyrin 3, MO22185, dilution 1:2,000 in green: [1] protein standard (red), [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, [6] SH-SY5Y cells, [7] rat brain, and [8] mouse brain lysates.