Product Details
Catalog Number: MO22187
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Dilutions: Immunofluorescence: 1:1,000 Immunohistochemistry: 1:1,000 Immunocytochemistry: 1:1,000 Western Blot: 1:10,000
Immunogen: Native NF-H purified from bovine spinal cord
Storage: Store at 4°C short term. Store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine, Equine
Entrez: 4744
UniProt: P12036
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ug of 1 mg/ml$275.00Add to Cart

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other proteins may also be present. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying axonal processes in tissue sections and in culture. NF-H antibodies can also be useful in visualizing neurofilament accumulations seen in many neurological disorders, such as Amyotrophic Lateral Sclerosis (also known as Lou Gehrig’s disease), Alzheimer’s disease and following a traumatic injury.

Images
Immunohistochemistry

Immunohistological analysis of rat cerebellum section stained with mouse mAb to pNF-H, MO22187, dilution 1:2,000 in green, and costained with rabbit pAb to FOX3/NeuN, dilution 1:5,000 in red. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies.

Immunohistochemistry

Immunohistological analysis of rat cerebellum section stained with mouse mAb to pNF-H, MO22187, dilution 1:2,000 in green, and costained with rabbit pAb to FOX3/NeuN, dilution 1:5,000 in red. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies.

Western Blot

Western blot detection of the heavily phosphorylated axonal form of NF-H protein (pNF-H) in neural tissue lysates (20µg/lane) with affinity purified mouse monoclonal anti-pNF-H antibody at dilution of 1:5,000. Lanes on the blot are: [1] Protein size marker, [2] Adult rat whole brain [3] Embryonic (E20) rat whole brain [4] Adult rat spinal cord [5] Adult mouse whole brain [6] Adult mouse spinal cord.

Western Blot

Western blot detection of the heavily phosphorylated axonal form of NF-H protein (pNF-H) in neural tissue lysates (20µg/lane) with affinity purified mouse monoclonal anti-pNF-H antibody at dilution of 1:5,000. Lanes on the blot are: [1] Protein size marker, [2] Adult rat whole brain [3] Embryonic (E20) rat whole brain [4] Adult rat spinal cord [5] Adult mouse whole brain [6] Adult mouse spinal cord.