The green fluorescent protein (GFP) is a 27kDa protein isolated originally from the jellyfish Aequoria victoria. It has an endogenous fluorochrome activity with excitation maximum at 395nm and emission maximum at 509nm, which is similar to that of fluorescein. The GFP gene was sequenced and the origin of the fluorochrome by autocatalytic activity of certain amino acids was discovered. Much interest in GFP was generated when it was shown that fluorescence develops rapidly when the protein is expressed and requires only molecular oxygen and no other cofactors. As a result GFP can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell. GFP has been engineered to produce a vast number of variously colored mutants including blue, cyan and yellow protein derivatives, BFP, CFP and YFP. GFP and other fluorescent proteins derived from other Cnidarians (jellyfish, coral and medusa) are widely used as tracers in transfection and transgenic experiments to monitor gene expression and protein localization in vivo and in in vitro. The crystal structure of GFP was determined which allowed amino acid modifications to improve spectral properties and prevent multimerization.
- Product Reviews
Leave a review for this product and you could be eligible for a $10 Starbucks gift card.
- Product Publications
If you've used this product in a publication, let us know. Email firstname.lastname@example.org, with the publication details and you could be eligible for an Amazon gift card.
- Related Products
Name Catalog # Size ATPase Na+/K+ transporting alpha 1 MO25023 50 ul Fibrillarin CH22129 100 ul Fibrillarin MO22169 100 µl Fibrillarin RA22147 100 ul Fibrillarin/Nop1p- Nucleolar Marker MO22108 500 ul FluoGreen Tracer SF41000 100 ul GFP GT22101 100 ul Green Fluorescent Protein CH22124 100 ul of 1 mg/ml Green Fluorescent Protein MO22184 100 ul Green Fluorescent Protein (GFP) RA22134 100 ul of 1 mg/ml High-mobility group protein B1/HMGP1 MO22134 100 ul Lamin A/C CH22136 100 ul Lamin A/C MO22124 100 ul mCherry GT22108 100 ul mCherry MO22192 100 ul mCherry - Mouse MO22140 100 ul mCherry-Chicken CH22115 100 ul mCherry-Rabbit RA22117 100 ul MiTF (Microphthalmia-Associated Transcription Factor) MO47034 0.5 ml Panspecific Nuclear Pore Complex Marker MO22107 500 ul S-Tag/S15 MO22203 100 ul S-Tag/S15 MO22206 100 ul TAF15 MO22114 100 ul Tar DNA Binding Protein 43 (TDP43) MO22117 100 ul