Product Details
Catalog Number: MO22192
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Dilutions: Immunohistochemistry: 1:1,500 Immunofluorescent: 1:1,500 Immunocytochemistry: 1:1,500 Western Blot: 1:2,000
Immunogen: Full length recombinant mCherry protein
Storage: Store at 4°C short term. Store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Species Reactivity: Not Applicable
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$295.00Add to Cart

The mCherry protein is derived from a natural product, DsRed, originally isolated as a red fluorescent protein from the coral of the genus Discosoma. As with other natural fluorescent proteins of Cnidarians (jelly fish, sea anemones and corals), the natural form of the protein forms stable tetramers in vivo. The protein is widely used as a fluorescent tracer in transfection, transgenic, photobleaching and FRET type experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is similar in size and general structural properties to GFP, but, obviously, produces a red rather than a green fluorochrome.

Images
Immunofluorescence

Immunofluorescent analysis of HEK293 cells stably transduced with a lentiviral vector expressing an mCherry-HA construct (red) and stained with mouse mAb to mCherry, dilution 1:500 in green.

Immunofluorescence

Immunofluorescent analysis of HEK293 cells stably transduced with a lentiviral vector expressing an mCherry-HA construct (red) and stained with mouse mAb to mCherry, dilution 1:500 in green.

Western Blot

Western blot analysis of various HEK293 cell lysates using mouse mAb to mCherry, dilution 1:5,000, in green. [1] protein standard, [2] untransfected HEK293 lysate, [3] lysate of HEK293 cells transfected with mCherry-HA construct, and [4] lysate of HEK293 cells transfected with an acGFP construct.

Western Blot

Western blot analysis of various HEK293 cell lysates using mouse mAb to mCherry, dilution 1:5,000, in green. [1] protein standard, [2] untransfected HEK293 lysate, [3] lysate of HEK293 cells transfected with mCherry-HA construct, and [4] lysate of HEK293 cells transfected with an acGFP construct.