Product Details
Catalog Number: MO22194
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Dilutions: Immunohistochemistry: 1:100-500 Immunofluorescent: 1:100-500 Immunocytochemistry: 1:100-500 Western Blot: 1:5,000-10,000
Immunogen: Full length native protein purified from pig spinal cord
Storage: Store at 4°C short term. Store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine, Equine
Entrez: 4747
UniProt: P07196
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ul$325.00Add to Cart

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins may be included also. The major function of neurofilaments is likely to control the diameter of large axons. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70kDa with some variability across species. Antibodies to NF-L like MCA-6H112 are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrig’s disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and others. Much interest has recently been focused on the detection of NF-L released from neurons into blood and CSF as a surrogate marker of primarily axonal loss in a variety of types of CNS injury and degeneration.

Images
Immunofluorescence

Immunofluorescent analysis of a section of mouse cerebellum stained with mouse mAb to NF-L dilution 1:5,000 in green, and costained with chicken pAb to FOX2 dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA.

Immunofluorescence

Immunofluorescent analysis of a section of mouse cerebellum stained with mouse mAb to NF-L dilution 1:5,000 in green, and costained with chicken pAb to FOX2 dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA.

Western Blot

Western blot analysis of different tissue lysates using mouse mAb to NF-L dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, and [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.

Western Blot

Western blot analysis of different tissue lysates using mouse mAb to NF-L dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, and [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.