Product Details
Catalog Number: MO22196
Applications: ICC, WB, IF
Type: Mouse IgG
Dilutions: Immunofluorescent: 1:1,000-2,000 Immunocytochemistry: 1:1,000-2,000 Western Blot: 1:1,000-2,000
Immunogen: Full length human PEA-15 as expressed in and purified from E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Bovine
Entrez: 8682
UniProt: Q15121
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$303.00Add to Cart

PEA-15 was originally isolated as a major low molecular weight of embryonic mouse striatal astrocytes grown in cell culture. Three spots on 2D gels with an apparent molecular weight of 15kDa and isoelectric point 5.1-5.3 were shown to be different forms of one protein. The protein was serine phosphorylated on one site by protein kinase C both in vivo and in vitro and the protein was named “phosphoprotein enriched in astrocytes of 15kDa”, hence PEA-15. Subsequent cloning and sequencing revealed a protein well conserved in sequence between mouse and human and which was heavily expressed in brain. Independently the same protein was found to be upregulated in fibroblasts and tissues of diabetic patients, and has hence named “protein enriched in diabetes” or PED. Immunocytochemical studies showed that the protein was heavily expressed in astrocytes and certain neurons in the CNS of mice, though it is expressed a lower levels ubiquitously. PEA-15 was shown to interact with extracellular signal regulated kinase and regulate the nuclear entry of this protein, and several other important interactions with other proteins involved in regulation of apoptosis, glucose metabolism and cell growth have been described. MCA-4D2 was made against a recombinant full length PEA-15 construct expressed in and purified from E. coli.

Images
Immunfluorescence

Immunofluorescent analysis of rat brain section stained with mouse mAb to PEA-15, MO22196, dilution 1:1,000 in red, and costained with chicken pAb to MAP2, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. The PEA-15 antibody labels the cytoplasm of certain presumably neuronal cells which are not labelled by the astrocyte specific GFAP antibody.

Immunfluorescence

Immunofluorescent analysis of rat brain section stained with mouse mAb to PEA-15, MO22196, dilution 1:1,000 in red, and costained with chicken pAb to MAP2, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. The PEA-15 antibody labels the cytoplasm of certain presumably neuronal cells which are not labelled by the astrocyte specific GFAP antibody.

Western Blot

Western blot analysis of different tissue lysates using mouse mAb to PEA-15 dilution 1:1,000 in green: [1] protein standard (red), [2] rat whole brain, [3] rat cerebellum, [4] mouse whole brain, [5] cow cortex and [6] cow cerebellum. The strong band at about 15kDa corresponds to the PEA-15 protein.

Western Blot

Western blot analysis of different tissue lysates using mouse mAb to PEA-15 dilution 1:1,000 in green: [1] protein standard (red), [2] rat whole brain, [3] rat cerebellum, [4] mouse whole brain, [5] cow cortex and [6] cow cerebellum. The strong band at about 15kDa corresponds to the PEA-15 protein.