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Immunofluorescent analysis of rat brain section stained with mouse mAb to PEA-15, MO22196, dilution 1:1,000 in red, and costained with chicken pAb to MAP2, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. The PEA-15 antibody labels the cytoplasm of certain presumably neuronal cells which are not labelled by the astrocyte specific GFAP antibody.
Immunofluorescent analysis of rat brain section stained with mouse mAb to PEA-15, MO22196, dilution 1:1,000 in red, and costained with chicken pAb to MAP2, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. The PEA-15 antibody labels the cytoplasm of certain presumably neuronal cells which are not labelled by the astrocyte specific GFAP antibody.
Western blot analysis of different tissue lysates using mouse mAb to PEA-15 dilution 1:1,000 in green: [1] protein standard (red), [2] rat whole brain, [3] rat cerebellum, [4] mouse whole brain, [5] cow cortex and [6] cow cerebellum. The strong band at about 15kDa corresponds to the PEA-15 protein.
Western blot analysis of different tissue lysates using mouse mAb to PEA-15 dilution 1:1,000 in green: [1] protein standard (red), [2] rat whole brain, [3] rat cerebellum, [4] mouse whole brain, [5] cow cortex and [6] cow cerebellum. The strong band at about 15kDa corresponds to the PEA-15 protein.