Neurofilament NF-L

Immunofluorescent analysis of cow cerebellum section stained with mouse mAb to NF-L, MO22201, dilution 1:2,000 in green, and costained with chicken pAb to VLP1 in red. Blue is Hoechst staining of nuclear DNA. MO22201 labels dendrites and axons of neuronal cells in the granular layer (lower left) and prominent basket cell axons surrounding the large Purkinje neurons. The VLP1 antibody reveals protein expressed in granule cells and in synapses of the molecular layer of the cerebellum.

Western blot analysis of different tissue lysates using mouse mAb to NF-L, MO22201, dilution 1:20,000 in green: [1] protein standard, [2] rat brain , [3] mouse brain and [4] cow cerebelum. Strong band at about 68-70kDa corresponds to NF-L protein, with the cow protein appearing slightly larger in molecular size as expected. Low molecular weight bands detected in cow brain sample are likely post mortem proteolytic forms of NF-L.