Product Details
Catalog Number: MO22202
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Dilutions: Immunofluorescence: 1:5,000; Immunohistochemistry: 1:5,000; Western Blot: 1:5,000-10,000
Immunogen: Pig brain tubulin preparation
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Primate
Entrez: 203068
UniProt: P07437
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

Tubulins are a major class of cytoskeletal proteins and divided into five distinct classes, namely α, β, γ, δ and ε. The most abundant members of this family are the α and β-tubulins which are the major components of cytoplasmic microtubules. The various subunits have molecular weights of approximately 50kDa and are 50% identical to one another at the protein sequence level. Microtubules are involved in a number of essential cellular functions including the maintenance of cell shape, vesicle and organelle transport, cell motility, cell signaling, meiosis and mitosis. The important role of microtubules in forming the mitotic spindle during cell division makes them a desirable target for the development of therapeutic agents directed against rapidly dividing cancer cells.

This antibody was raised against tubulin purified from pig brain. Antibodies to β-tubulin are widely used as loading controls in western blotting experiments as a standard by which the levels of other proteins may be measured.

Images
Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with mouse mAb to β-tubulin, MO22202, dilution 1:5,000 in green, and costained with chicken pAb to the nucleic acid binding protein FOX2 in red. Blue is DAPI staining of nuclear DNA. MO22202 antibody produces strong staining of microtubules in the cytoplasm, while the FOX2 antibody labels the nuclei of HeLa cells.

Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with mouse mAb to β-tubulin, MO22202, dilution 1:5,000 in green, and costained with chicken pAb to the nucleic acid binding protein FOX2 in red. Blue is DAPI staining of nuclear DNA. MO22202 antibody produces strong staining of microtubules in the cytoplasm, while the FOX2 antibody labels the nuclei of HeLa cells.

Western Blot

Western blot analysis of whole tissue or cell lysates using mouse mAb to β-tubulin, MO22202, dilution 1:10,000 in green: [1] protein standard (red), [2] adult rat brain, [3] adult mouse brain, [4] NIH-3T3 cells, [5] HEK293 cells, [6] HeLa cells, [7] SH-SY5Y cells. The strong clean band with apparent SDS-PAGE molecular weight of ~50kDa corresponds to the β-tubulin proteins.

Western Blot

Western blot analysis of whole tissue or cell lysates using mouse mAb to β-tubulin, MO22202, dilution 1:10,000 in green: [1] protein standard (red), [2] adult rat brain, [3] adult mouse brain, [4] NIH-3T3 cells, [5] HEK293 cells, [6] HeLa cells, [7] SH-SY5Y cells. The strong clean band with apparent SDS-PAGE molecular weight of ~50kDa corresponds to the β-tubulin proteins.