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Images
Western blot analysis of 0.25g of different recombinant proteins solutions expressed in pET family vectors and which contain the S-tag sequence (KETAAAKFERQHMDS), using mouse mAb MO22203, dilution 1:5,000 in green: [1] protein standard (red), [2] aldolase A, [2] MBP, [4] MAP2, P2 projection domain construct and [5] FOX2-C terminal part of the protein. MO22203 binds to the S-tag present in all tested recombinant proteins, and reveals protein bands of the expected molecular weights.
Western blot analysis of 0.25g of different recombinant proteins solutions expressed in pET family vectors and which contain the S-tag sequence (KETAAAKFERQHMDS), using mouse mAb MO22203, dilution 1:5,000 in green: [1] protein standard (red), [2] aldolase A, [2] MBP, [4] MAP2, P2 projection domain construct and [5] FOX2-C terminal part of the protein. MO22203 binds to the S-tag present in all tested recombinant proteins, and reveals protein bands of the expected molecular weights.
Western blot analysis of bovine pancreas tissue lysate using mouse mAb to S-tag protein, MO22203, dilution 1:2,000 in green: [1] protein standard (red), [2] 40µg of bovine pancreas extract. A strong band at about 15kDa corresponds to S-tag sequence found in bovine pancreatic RNase 1.
Western blot analysis of bovine pancreas tissue lysate using mouse mAb to S-tag protein, MO22203, dilution 1:2,000 in green: [1] protein standard (red), [2] 40µg of bovine pancreas extract. A strong band at about 15kDa corresponds to S-tag sequence found in bovine pancreatic RNase 1.