Product Details
Catalog Number: MO22203
Applications: ICC, WB, IF
Type: Mouse IgG
Dilutions: Immunofluorescence: N/A; Western Blot: 1:2,000-5,000
Immunogen: Peptide derived from the N-terminal sequence of pET30a(+)
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Bovine
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

The S-tag is one of many intrinsically unstructured peptides which only adopts a defined structure on binding to a structured substrate. The S-tag is incorporated into many vectors including the pET29 and 30 series, pCITE-3 and pCITE-4. The S-tag sequence was incorporated into many expression systems and is detectable with certain antibody reagents allowing researchers to check the size and correct expression of recombinant proteins. Proteins including the S-tag can be purified using a column to which is bound the RNAse-S-prot. The modern nomenclature for the enzyme is RNAase A or RNAse 1.

This antibody was made against a synthetic 53 amino acid peptide which is the sequence included in pET30a(+) and other vectors. A C-terminal cysteine was added to allow coupling to maleate activated KLH which was used as the immunogen. Numerous clones were screened by their ability to bind the immunogen and then re-screened for inhibition of this binding by the S-tag peptide.

Images
Western Blot 1

Western blot analysis of 0.25g of different recombinant proteins solutions expressed in pET family vectors and which contain the S-tag sequence (KETAAAKFERQHMDS), using mouse mAb MO22203, dilution 1:5,000 in green: [1] protein standard (red), [2] aldolase A, [2] MBP, [4] MAP2, P2 projection domain construct and [5] FOX2-C terminal part of the protein. MO22203 binds to the S-tag present in all tested recombinant proteins, and reveals protein bands of the expected molecular weights.

Western Blot 1

Western blot analysis of 0.25g of different recombinant proteins solutions expressed in pET family vectors and which contain the S-tag sequence (KETAAAKFERQHMDS), using mouse mAb MO22203, dilution 1:5,000 in green: [1] protein standard (red), [2] aldolase A, [2] MBP, [4] MAP2, P2 projection domain construct and [5] FOX2-C terminal part of the protein. MO22203 binds to the S-tag present in all tested recombinant proteins, and reveals protein bands of the expected molecular weights.

Western Blot 2

Western blot analysis of bovine pancreas tissue lysate using mouse mAb to S-tag protein, MO22203, dilution 1:2,000 in green: [1] protein standard (red), [2] 40µg of bovine pancreas extract. A strong band at about 15kDa corresponds to S-tag sequence found in bovine pancreatic RNase 1.

Western Blot 2

Western blot analysis of bovine pancreas tissue lysate using mouse mAb to S-tag protein, MO22203, dilution 1:2,000 in green: [1] protein standard (red), [2] 40µg of bovine pancreas extract. A strong band at about 15kDa corresponds to S-tag sequence found in bovine pancreatic RNase 1.