The S-tag is one of many intrinsically unstructured peptides which only adopts a defined structure on binding to a structured substrate. The S-tag is incorporated into many vectors including the pET29 and 30 series, pCITE-3 and pCITE-4. The S-tag sequence was incorporated into many expression systems and is detectable with certain antibody reagents allowing researchers to check the size and correct expression of recombinant proteins. Proteins including the S-tag can be purified using a column to which is bound the RNAse-S-prot. The modern nomenclature for the enzyme is RNAase A or RNAse 1.
This antibody was made against a synthetic 53 amino acid peptide which is the sequence included in pET30a(+) and other vectors. A C-terminal cysteine was added to allow coupling to maleate activated KLH which was used as the immunogen. Numerous clones were screened by their ability to bind the immunogen and then re-screened for inhibition of this binding by the S-tag peptide.
- Product Reviews
Leave a review for this product and you could be eligible for a $10 Starbucks gift card.
- Product Publications
If you've used this product in a publication, let us know. Email firstname.lastname@example.org, with the publication details and you could be eligible for an Amazon gift card.
- Related Products
Name Catalog # Size ATPase Na+/K+ transporting alpha 1 MO25023 50 ul Fibrillarin CH22129 100 ul Fibrillarin MO22169 100 µl Fibrillarin RA22147 100 ul Fibrillarin/Nop1p- Nucleolar Marker MO22108 500 ul FluoGreen Tracer SF41000 100 ul GFP GT22101 100 ul Green Fluorescent Protein CH22124 100 ul of 1 mg/ml Green Fluorescent Protein MO22184 100 ul Green Fluorescent Protein (GFP) MO22190 100 ul Green Fluorescent Protein (GFP) RA22134 100 ul of 1 mg/ml High-mobility group protein B1/HMGP1 MO22134 100 ul Lamin A/C CH22136 100 ul Lamin A/C MO22124 100 ul mCherry GT22108 100 ul mCherry MO22192 100 ul mCherry - Mouse MO22140 100 ul mCherry-Chicken CH22115 100 ul mCherry-Rabbit RA22117 100 ul MiTF (Microphthalmia-Associated Transcription Factor) MO47034 0.5 ml Panspecific Nuclear Pore Complex Marker MO22107 500 ul S-Tag/S15 MO22206 100 ul TAF15 MO22114 100 ul Tar DNA Binding Protein 43 (TDP43) MO22117 100 ul