Product Details
Catalog Number: MO22204
Applications: ICC, WB, IHC, IF
Type: Mouse IgM
Dilutions: Immunofluorescence: 1:100; Immunohistochemistry: 1:100; Western Blot: 1:1,000
Immunogen: Full length protein purified from pig blood cells
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Primate, Chicken, Bovine, Dog, Porcine, Equine
Entrez: 2597
UniProt: P00355
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate to produce glyceraldehyde 1-3-bisphosphate. Because GAPDH protein is expressed in large amounts and is required at all times for an important “house keeping” function, levels of GAPDH mRNA are often measured and used as standards in studies of the expression of other mRNAs. Similarly specific antibodies to GAPDH are used to measure GAPDH expression as a protein standard in western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue.

This antibody was raised against extensively purified pig GAPDH. The antibody has been widely used as a western blotting standard by many labs for many years and is known to detect GAPDH in a variety of mammalian species including human, rat and mouse. Current data suggests that the epitope for the antibody is within the peptide KYDDIKKVVKQASEGPLKGI, amino acids 254-273 of the human sequence, a peptide which is highly conserved across mammalian species. The antibody produces a single clean band on western blots of typical cell and tissue extracts.

Images
Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with mouse mAb to GAPDH, MO22204, dilution 1:100 in green. Blue is Hoechst staining of nuclear DNA. MO22204 produces strong cytoplasmic staining of healthy cells.

Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with mouse mAb to GAPDH, MO22204, dilution 1:100 in green. Blue is Hoechst staining of nuclear DNA. MO22204 produces strong cytoplasmic staining of healthy cells.

Western Blot

Western blot analysis of cell line lysates probed with mouse mAb to GAPDH, MO22204, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a “house keeping” protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blot loading control.

Western Blot

Western blot analysis of cell line lysates probed with mouse mAb to GAPDH, MO22204, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a “house keeping” protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blot loading control.