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Immunofluorescent analysis of HeLa cells stained with mouse mAb to GAPDH, MO22204, dilution 1:100 in green. Blue is Hoechst staining of nuclear DNA. MO22204 produces strong cytoplasmic staining of healthy cells.
Immunofluorescent analysis of HeLa cells stained with mouse mAb to GAPDH, MO22204, dilution 1:100 in green. Blue is Hoechst staining of nuclear DNA. MO22204 produces strong cytoplasmic staining of healthy cells.
Western blot analysis of cell line lysates probed with mouse mAb to GAPDH, MO22204, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a “house keeping” protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blot loading control.
Western blot analysis of cell line lysates probed with mouse mAb to GAPDH, MO22204, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a “house keeping” protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blot loading control.