Product Details
Catalog Number: MO22205
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Dilutions: Immunofluorescence: 1:500; Immunohistochemistry: 1:500; Western Blot: 1:1,000
Immunogen: Recombinant human alpha-helical GFAP fragment expressed in and purified from E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine
Entrez: 2670
UniProt: P14136
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

Glial fibrillary acidic protein (GFAP) is strongly and specifically expressed in astrocytes, Bergmann glia, certain other glia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. GFAP expression is also seen in developing neural stem cells and GFAP levels may greatly increase in regions of CNS injury or disease. The formation of a GFAP rich “glial scar” following CNS injury may be one reason why reconnection of severed processes is relatively inefficient in adults.

This antibody was made against a recombinant construct containing amino acids 71-217 of the human isotype 1 sequence in NP_002046.1. This region is somewhat variable between species so antibodies to this human construct may be superior on human cells, tissues and for biomarker assays of human proteins. MO22205 has a KD of 6.157 X 10-10M. High quality antibodies to GFAP, like MO22205, are useful for visualizing glia and monitoring developmental, disease and damage related CNS alterations and for ELISA and bead based type assays.

Images
Immunofluorescence

Immunofluorescent analysis of rat hippocampus section stained with mouse mAb to GFAP, MO22205, dilution 1:500 in red, and costained with chicken pAb to MAP2 in green. The blue is Hoechst staining of nuclear DNA. The GFAP antibody stains the network of astroglial cells while the MAP2 antibody labels perikarya and dendrites of neurons.

Immunofluorescence

Immunofluorescent analysis of rat hippocampus section stained with mouse mAb to GFAP, MO22205, dilution 1:500 in red, and costained with chicken pAb to MAP2 in green. The blue is Hoechst staining of nuclear DNA. The GFAP antibody stains the network of astroglial cells while the MAP2 antibody labels perikarya and dendrites of neurons.

Western Blot

Western blot analysis of equal amount of total proteins from different cell and tissue lysates using mouse mAb to GFAP, MO22205, dilution 1:1,000 in green: [1] protein standard (red), [2] primary rat cortical neuron-glial cells, [3] rat brain, [4] mouse brain, [5] cow cortex, [6] cow cerebellum and [7] pig hippocampus. Bands around 50kDa marks correspond to GFAP protein and its alternative transcripts and fragments.

Western Blot

Western blot analysis of equal amount of total proteins from different cell and tissue lysates using mouse mAb to GFAP, MO22205, dilution 1:1,000 in green: [1] protein standard (red), [2] primary rat cortical neuron-glial cells, [3] rat brain, [4] mouse brain, [5] cow cortex, [6] cow cerebellum and [7] pig hippocampus. Bands around 50kDa marks correspond to GFAP protein and its alternative transcripts and fragments.