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Immunofluorescent analysis of HeLa cells stained with mouse mAb to annexin A6, MO22207, dilution 1:500 in red, and costained with chicken pAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. The annexin A6 antibody detects protein predominantly expressed in spherical cellular membrane protrusions, known as blebs, while the vimentin antibody produces strong staining of theintermediate filament network of the cytoskeleton.
Immunofluorescent analysis of HeLa cells stained with mouse mAb to annexin A6, MO22207, dilution 1:500 in red, and costained with chicken pAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. The annexin A6 antibody detects protein predominantly expressed in spherical cellular membrane protrusions, known as blebs, while the vimentin antibody produces strong staining of theintermediate filament network of the cytoskeleton.
Western blot analysis of different cell lysates using mouse mAb to annexin A6, MO22207, dilution 1:2,000 in green: [1] protein standard (red), [2] mouse NIH-3T3, [3] rat C6, [4] human HeLa, [5] human HEK293, [6] dog A72, [7] equine NBL6 and [8] African green monkey COS1 cells. MCA-4G3 antibody detects annexin A6 protein with apparent MW of about 68kDa in all cell preparations. The blot was simultaneously probed with rabbit pAb to HSP60. The band at 60kDa corresponds to HSP60 protein.
Western blot analysis of different cell lysates using mouse mAb to annexin A6, MO22207, dilution 1:2,000 in green: [1] protein standard (red), [2] mouse NIH-3T3, [3] rat C6, [4] human HeLa, [5] human HEK293, [6] dog A72, [7] equine NBL6 and [8] African green monkey COS1 cells. MCA-4G3 antibody detects annexin A6 protein with apparent MW of about 68kDa in all cell preparations. The blot was simultaneously probed with rabbit pAb to HSP60. The band at 60kDa corresponds to HSP60 protein.