Product Details
Catalog Number: MO22207
Applications: ICC, WB, IF
Type: Mouse IgG
Dilutions: Immunofluorescence: 1:1,000; Western Blot: 1:1,000-2,000
Immunogen: Recombinant full length annexin A6 expressed in and purified from E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Mouse
Entrez: 309
UniProt: P08133
Downloads: Datasheet (pdf)
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100 ul$275.00Add to Cart

The annexins are a large family of related proteins which share the property of binding to phophospholipid containing membranes in a Calcium dependent manner. Annexin A6 has a particularly surprising number of alternate names, 19 being listed on the Genecards ANXA6 site Annexin A6 is normally localized in the cytosol but becomes membrane associated following cellular injury, and so is often seen in ““blebs”, transient herniations of the plasma membrane associated with apoptosis and more generally indicative of cell stress. Recent studies suggest that annexin A6 functions, in combination with annexin A4, in the repair of cell membranes following cellular injury.

This antibody was made against full length recombinant human annexin A6 expressed in and purified from E. coli. The antibody recognizes annexin A6 in in human, rodents and many other mammals and can be used as a marker of blebs, indicative of cellular stress.

Images
Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with mouse mAb to annexin A6, MO22207, dilution 1:500 in red, and costained with chicken pAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. The annexin A6 antibody detects protein predominantly expressed in spherical cellular membrane protrusions, known as blebs, while the vimentin antibody produces strong staining of theintermediate filament network of the cytoskeleton.

Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with mouse mAb to annexin A6, MO22207, dilution 1:500 in red, and costained with chicken pAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. The annexin A6 antibody detects protein predominantly expressed in spherical cellular membrane protrusions, known as blebs, while the vimentin antibody produces strong staining of theintermediate filament network of the cytoskeleton.

Western Blot

Western blot analysis of different cell lysates using mouse mAb to annexin A6, MO22207, dilution 1:2,000 in green: [1] protein standard (red), [2] mouse NIH-3T3, [3] rat C6, [4] human HeLa, [5] human HEK293, [6] dog A72, [7] equine NBL6 and [8] African green monkey COS1 cells. MCA-4G3 antibody detects annexin A6 protein with apparent MW of about 68kDa in all cell preparations. The blot was simultaneously probed with rabbit pAb to HSP60. The band at 60kDa corresponds to HSP60 protein.

Western Blot

Western blot analysis of different cell lysates using mouse mAb to annexin A6, MO22207, dilution 1:2,000 in green: [1] protein standard (red), [2] mouse NIH-3T3, [3] rat C6, [4] human HeLa, [5] human HEK293, [6] dog A72, [7] equine NBL6 and [8] African green monkey COS1 cells. MCA-4G3 antibody detects annexin A6 protein with apparent MW of about 68kDa in all cell preparations. The blot was simultaneously probed with rabbit pAb to HSP60. The band at 60kDa corresponds to HSP60 protein.