Product Details
Catalog Number: MO22208
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Dilutions: Immunofluorescence: 1:1,000; Immunohistochemistry: 1:1,000; Western Blot: 1:10,000
Immunogen: Full length MAP2A/B purified from bovine spinal cord
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Bovine
Entrez: 4133
UniProt: P11137
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells and are associated with a family of proteins called microtubule associated proteins (MAPs). MAPs play a crucial role in the regulation of microtubule dynamics and interactions in vivo. MAP2 was originally named as one of the higher molecular weight MAPs with an SDS-PAGE molecular weight of about 280kDa. MAP2 isoforms are expressed only in neurons, specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 isotypes are therefore excellent markers of neuronal dendrites and are useful for identifying neurons in cell culture and sections.

This antibody was raised against purified full length bovine brain MAP2 and the epitope was mapped to amino acids 631-1056 of the human sequence.

Images
Immunofluorescence

Immunofluorescent analysis of a rat hippocampus section stained with mouse mAb to MAP2, MO22208, dilution 1:2,000 in green, and costained with rabbit pAb to FOX3/NeuN in red. MO22208 labels MAP2 protein in the perikarya and dendrites of most neurons while the FOX3/NeuN antibody selectively stains nuclei and proximal soma of neuronal cells.

Immunofluorescence

Immunofluorescent analysis of a rat hippocampus section stained with mouse mAb to MAP2, MO22208, dilution 1:2,000 in green, and costained with rabbit pAb to FOX3/NeuN in red. MO22208 labels MAP2 protein in the perikarya and dendrites of most neurons while the FOX3/NeuN antibody selectively stains nuclei and proximal soma of neuronal cells.

Western Blot

Western blot analysis of tissue and cell lysates using mouse mAb to MAP2, MO22208, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, and [4] embryonic rat cortical neuron-glial cell lysate. A band at about 280 kDa corresponds to the MAP2A and MAP2B proteins.

Western Blot

Western blot analysis of tissue and cell lysates using mouse mAb to MAP2, MO22208, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, and [4] embryonic rat cortical neuron-glial cell lysate. A band at about 280 kDa corresponds to the MAP2A and MAP2B proteins.