The histochemical antibody for tyrosine hydroxylase (TH) is a mouse monoclonal antibody generated against TH that has been isolated and purified from rat PC12 cells. The antibody is believed to have wide species cross-reactivity because it recognizes an epitope in the mid-portion of the TH molecule where extensive species homology exists.
This Tyrosine Hydroxylase antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat catecholamine neuron systems using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions for these methods are provided on the data-sheet. This antibody does not cross react with dihydropterdine reductase, dopamine-B-hydroxylase, phenylethanolamine-N-methyltransferase, phenylalanine hydroxylase or tryptophan hydroxylase using Western blot methods.
Image: TH Staining in the Substantia Nigra.
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- Raju, Bina; Hultström, Michael; Haug, Sivakami R.; Ibrahim, Salah O.; Heyeraas, Karin J. (2009). Sympathectomy suppresses tumor growth and alters gene-expression profiles in rat tongue cancer. European Journal of Oral Sciences, Volume 117(4), August 2009, p 351-361. doi: 10.1111/j.1600-0722.2009.00646.x
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