Product Details
Catalog Number: MO25017
Applications: ICC, WB, IF
Type: Mouse IgG
Immunogen: Purified recombinant rat alpha-internexin expressed in and purified from E. coli. Specifically recognizes the C-terminal non-helical extension of a-internexin.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Supernatant
Format B: liquid
Species Reactivity: Human, Rat
Entrez: 9118
UniProt: Q16352
Downloads: Datasheet (pdf)
Product Sizes
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500 ul$275.00Add to Cart

Alpha-Internexin is a ~66 kDa Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M, and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M, and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest.

Images

Mixed cultures of rat CNS cells stained with alpha-Internexin (red) and chicken antibody to Microtubule associated protein 2 (MAP2- green). The a-internexin is localized primarily in neuronal axons in these cultures, while the perikarya and dendrites of neurons stain strongly for MAP2.

Mixed cultures of rat CNS cells stained with alpha-Internexin (red) and chicken antibody to Microtubule associated protein 2 (MAP2- green). The a-internexin is localized primarily in neuronal axons in these cultures, while the perikarya and dendrites of neurons stain strongly for MAP2.