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Images
Human neuroblastma line Sh-SY5Y stained with anti-GAPDH (green) and NF-H (red), counterstained with a fluorescent DNA probe (blue).
Human neuroblastma line Sh-SY5Y stained with anti-GAPDH (green) and NF-H (red), counterstained with a fluorescent DNA probe (blue).
Sciatic nerves of mouse wild type (+/+), heterozygous (+/o) and homozygous (o/o) for knock out of peripheral myelin protein 21 (pmp21) were homogenized in SDS-PAGE sample buffer and run out for GAPDH western blots. Dilution was 1:1000. Signals were revealed in a few seconds with chemiluminescence, indicating that lower antibody concentrations would also have worked well. Note the sharp clear band at 38kDa, the expected molecular weight for GAPDH.
Sciatic nerves of mouse wild type (+/+), heterozygous (+/o) and homozygous (o/o) for knock out of peripheral myelin protein 21 (pmp21) were homogenized in SDS-PAGE sample buffer and run out for GAPDH western blots. Dilution was 1:1000. Signals were revealed in a few seconds with chemiluminescence, indicating that lower antibody concentrations would also have worked well. Note the sharp clear band at 38kDa, the expected molecular weight for GAPDH.