Astrocyte-Growth Media is freshly prepared by mixing two components: 450 ml of Astrocyte Basal Media and 50 ml of Astrocyte-Growth supplement.
Astrocyte Basal Media: Astrocyte Basal Medium is basal media designed for optimal growth of normal human brain astrocytes in vitro in conjunction with 0.5% fetal bovine serum (FBS). Astrocyte basal medium is sterile liquid medium that contains essential and non-essential amino acids, vitamins, organics and inorganic compounds and trace minerals. It does not contain antimycotics, antibiotics, hormones, growth factors or proteins. It is bicarbonate buffered has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air
Astrocyte-Growth Supplement: Contains the essential nutrients for astrocyte cell proliferation; including 10% fetal bovine serum, recombinant growth factors and 1x penicillin and streptomycin
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