AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
AFP produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 597 amino acids (19-609a.a.) and having a molecular mass of 67.3kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
AFP is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
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