Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor-dependent cell lines and promotion of megakaryocyte production in vivo. Mouse LIF is a 20 kDa protein containing 181 amino acid residues.
Our ISOkine™ LIF is produced using barley grain as a bioreactor, and therefore is animal-free, serum-free and endotoxin-free. Furthermore, our unique technology enables us to offer high quality human LIF at much lower prices than the market leaders.
Testimonial: Bioactivity was tested by culturing mouse ES cells either on feeders in complete medium or without feeders in a conditioned medium. In both conditions ISOkine™ LIF was added using concentrations down to 0.1 ng/ml. All concentration conditions resulted in nice looking colonies of pluripotent mouse ES cells. When the ES cells were deprived of LIF they spontaneously differentiated and were able to form embryoid bodies in hanging drops. Dr. Guðrún Valdimarsdóttir, Dept. of Biochemistry and Molecular Biology, Faculty of Medicine, University of Iceland.
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