Product Details
Catalog Number: PR80004
Applications: Cell Assays
Type: Protein
Storage: Store at 4°C short term. Store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Ambient
Format B: lyophilized
Species Reactivity: Human
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
1 mg$3,118.00Add to Cart
10 ug$155.00Add to Cart
100 ug$555.00Add to Cart

Flt3-ligand (FMS related tyrosine kinase 3 ligand, flk-2 ligand) is involved in proliferation and differentiation of early hematopietic cells. Flt3-ligand synergizes with other CSF’s and interleukins to induce proliferation of early hematopoietic cells, but does not stimulate growth or differentation alone. Flt3-ligand binds to cells expressing the tyrosine kinase receptor Flt3. Multiple isoforms of Flt3-ligand have been identified but the predominant biologically active isoform is a transmembrane protein which can be proteolytically cleaved to generate a biologically active soluble form.

Our ISOkine™ Flt3-Ligand is  produced using barley grain as a bioreactor, and therefore is animal-free, serum-free and endotoxin-free. Furthermore our unique technology enables us to offer high quality human Flt-3 ligand at much lower prices than the market leaders.

Testimonial: Bioactivity was tested by culturing mouse ES cells either on feeders in complete medium or without feeders in a conditioned medium. In both conditions ISOkine™ LIF was added using concentrations down to 0.1 ng/ml. All concentration conditions resulted in nice looking colonies of pluripotent mouse ES cells. When the ES cells were deprived of LIF they spontaneously differentiated and were able to form embryoid bodies in hanging drops. Dr. Guðrún Valdimarsdóttir, Dept. of Biochemistry and Molecular Biology, Faculty of Medicine, University of Iceland.

Images

Flt3-Ligand IsoKINETM Tested for bioactivity vs leading E. Coli derived growth factor

Flt3-Ligand IsoKINETM Tested for bioactivity vs leading E. Coli derived growth factor

Plant cell system: The barley plant does not contain any pathogens or other components known to be harmful for human or animal stem cells. The plant components are safe and inert. Bioassay analysis and years of successful application in stem cell research verify this further.

Plant cell system: The barley plant does not contain any pathogens or other components known to be harmful for human or animal stem cells. The plant components are safe and inert. Bioassay analysis and years of successful application in stem cell research verify this further.