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- Brigit High, Marie E. Jetté, Mei Li, Vijay R. Ramakrishnan, Matthew Clary, Jeremy Prager, Julia Draf, Thomas Hummel, and Thomas E. Finger. (2023). Variability in P2X Receptor Composition in Human Taste Nerves: Implications for Treatment of Chronic Cough. ERJ Open Research. doi: 10.1183/23120541.00007-2023
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Western blot analysis indicated that concatamerized trimers remain intact. Total protein from oocytes injected with RNA for wild type P2X2 monomer, or trimer HH-HH-HH, HA-AA-AH, and AA-AA-HH was separated by SDS-PAGE under reducing conditions on a 4–12% gradient gel along with protein from uninjected oocytes. DOI 10.1074/jbc.M504545200.
Western blot analysis indicated that concatamerized trimers remain intact. Total protein from oocytes injected with RNA for wild type P2X2 monomer, or trimer HH-HH-HH, HA-AA-AH, and AA-AA-HH was separated by SDS-PAGE under reducing conditions on a 4–12% gradient gel along with protein from uninjected oocytes. DOI 10.1074/jbc.M504545200.