Catalog Number: RA18011
Applications: WB, IHC, IF
Type: Rabbit IgG
Immunogen: Phosphopeptide corresponding to residues around serine 890 of human NMDAR1
Storage: Do not aliquot. Store at -20°C. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat
N-methyl-D-aspartate receptor (NMDAR) forms a heterodimer of at least one NR1 and one NR2A-D subunit. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits. NR1 subunits bind the co-agonist glycine and NR2 subunits bind the neurotransmitter glutamate. Activation of the NMDA receptor or opening of the ion channel allows flow of Na+ and Ca2+ ions into the cell, and K+ out of the cell (1). Each subunit has a cytoplasmic domain that can be directly modified by the protein kinase/phosphatase (2). PKC can phosphorylate the NR1 subunit (NMDAR1) of the receptor on Ser890/Ser896, and PKA can phosphorylate NR1 on Ser897 (3). The phosphorylation of NR1 by PKC decreases its affinity for calmodulin, thus preventing the inhibitory effect of calmodulin on NMDAR (4). The phosphorylation of NR1 by PKA probably counteracts the inhibitory effect of calcineurin on the receptor (5). NMDAR mediates long-term potentiation and slow postsynaptic excitation, which play central roles in learning, neurodevelopment and neuroplasticity (6).
Liu, X.B. et al. (2004) J. Neurosci. 24, 8885-8895. Westphal, R.S. et al. (1999) Science 285, 93-96. Tingley, W.G. et al. (1997) J. Biol. Chem. 272, 5157-5166. Hisatsune, C. et al. (1997) J. Biol. Chem. 272, 20805-20810. Raman, I.M. et al. (1996) Neuron 16, 415-421. Makhinson, M. et al. (1999) J. Neurosci. 19, 2500-2510.
- Product Reviews
- Product Publications
If you've used this product in a publication, let us know. Email firstname.lastname@example.org, with the publication details and you could be eligible for an Amazon gift card.
- Related Products
Name Catalog # Size GABA A receptor alpha 4 GT41008 100 ug GABA A Receptor beta 3 GT41011 100 ug GABA A receptor, pi subunit RA30047 50 ug GABA Receptor Associated Protein RA19014 100 ug GluR2/AMPA2 RA30012 50 ug GluR4/AMPA4 GT41012 100 ug Nicotinic acetylcholine receptor alpha7 GT41009 100 ug NMDA NR1 Pan MO25041 15 ug NMDA Receptor 1, N1 RA25036 25 ug NMDA Receptor 2A RA25037 100 ul NMDA Receptor 2B RA25063 10 ug P2X1 RA10107 150 ul P2X1 RA10107 50 ul P2X2 GP14106 150 ul P2X2 GP14106 50 ul P2X2 RA10108 150 ul P2X2 RA10108 50 ul P2X3 GP10108 100 ul P2X3 RA10109 150 ul P2X3 RA10109 50 ul P2X3-Pure RA14139 100 ul Phospho-NMDA Receptor 1 (Ser896) RA18012 100 ul