100% homology with human and mouse.
The biological activities of the neurotrophins are mediated by binding to the different members of the Trk family tyrosine kinase receptors. Three Trk family proteins, TrkA, TrkB and TrkC, exhibiting different ligand specificities, have been identified. At the protein sequence level, Trks are highly conserved between species and the proteins exhibit cross-species activity.
Specificity/Sensitivity: Phospho-TrkA (Tyr490) Antibody detects endogenous levels of Trk only when phosphorylated at tyrosine 490. This antibody also detects TrkB and TrkC when phosphorylated at the corresponding residues.
Images: Increased mobilization of trkA to neuronal membrane enhances response to NGF challenge. A, Fluorescence photomicrographs depicting relative levels of activated/phosphorylated trkA (phospho-trkA) detected under permeabilizing conditions in representative adult DRG neurons exposed to acidic (pH 6.5) or control (pH 7.4) media (in the presence of anti-BDNF) for 30 min, followed by a 15 min challenge with pH-specific medium + anti-BDNF alone or with anti-BDNF + 50 ng/ml NGF. Scale bar, 10 μm. Scatter plots illustrate the relationship between phospho-trkA-like labeling intensity (y-axis; normalized to the mean signal intensity from the control pH group) and perikaryal diameter (x-axis) for all sensory neurons analyzed from three separate experiments exposed to control (pH 7.4, B) or acidic (pH 6.5, C) media as above and then challenged with 50 ng/ml NGF (open circles) or not (filled circles). D, Summary bar graph depicting relative levels of activated trkA (phospho-trkA; normalized to pH 7.4 control) in representative adult DRG neurons exposed to acidic (pH 6.5) or control (pH 7.4) media for 30 min and then challenged with 50 ng/ml NGF and as normalized to the mean signal intensity from the control pH group. E, Fluorescence photomicrographs of sensory neurons exposed to identical conditions as in A and processed to detect activated/phosphorylated p38MAPK Note: A significant increase is observed in the levels of phospho-trkA detected in sensory neurons in response to acidosis with a parallel response observed for phospho-p38MAPK (E) (one-way ANOVA with post hoc Tukey's; ***p < 0.001; n = 131–210 neurons analyzed per experimental condition). Scale bar, 20 μm. The Journal of Neuroscience, 8 May 2013, 33(19): 8202-8215; doi: 10.1523/JNEUROSCI.4408-12.2013-phospho-TrkA Customer Publications.
- Product ReviewsPlaced the order on Aug. 21st received the product on August 23rd. Prompt service coming from the U.S.! Product Name: Phospho-TrkA (Tyr490), rabbit – (Cat# RA18018) http://bit.ly/2flbZqq Organization: University of SaskatchewanShannon - Sep 14, 2017 - Rating: 5.0
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- Product Publications
- Geoffrey E. Bray, Zhengxin Ying, Landon D. Baillie, Ruiling Zhai, Sean J. Mulligan, and Valerie M.K. Verge. (2013). Extracellular pH and Neuronal Depolarization Serve as Dynamic Switches to Rapidly Mobilize trkA to the Membrane of Adult Sensory Neurons. The Journal of Neuroscience, 33(19):8202-8215. doi: 10.1523/JNEUROSCI.4408-12.2013
- Yuan Lu, Yuanyuan Ji, Sundar Ganesan, Robert Schloesser, Keri Martinowich, Mu Sun, Fan Mei, Moses V. Chao, and Bai Lu. (2011). TrkB as a Potential Synaptic and Behavioral Tag. The Journal of Neuroscience, 31(33): 11762-11771. doi: 10.1523/JNEUROSCI.2707-11.2011
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