Product Details
Catalog Number: RA18030
Applications: WB, IHC, FC, IF
Type: Rabbit IgG
Immunogen: synthetic peptide (KLH-coupled) surrounding Asp85 of human HSP60.
Storage: Do not aliquot. Store at -20°C. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Primate
Entrez: 3329
UniProt: P10809
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ul$332.00Add to Cart

Hsp60 is a member of a highly conserved family which includes molecular chaperones. Consistent with their functions as chaperones, Hsp60 and Hsp10 have been suggested to act as docking molecules with a passive role in the maturation of caspase processing, and are primarily known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria . It is now clear that a significant amount of HSP60 is also present in the cytosol of many cells and that it is induced by stress, inflammatory and immune responses, autoantibodies correlated with Alzheimer's, coronary artery diseases, MS, and diabetes.

Images

Confocal immunofluorescent analysis of HeLa cells using HSP60 (D85) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Confocal immunofluorescent analysis of HeLa cells using HSP60 (D85) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Western blot analysis of extracts from various cell types using HSP60 (D85) Antibody.

Western blot analysis of extracts from various cell types using HSP60 (D85) Antibody.

Flow cytometric analysis of HeLa cells using HSP60 (D85) Antibody (blue) compared to a nonspecific negative control antibody (red).

Flow cytometric analysis of HeLa cells using HSP60 (D85) Antibody (blue) compared to a nonspecific negative control antibody (red).