Product Details
Catalog Number: RA20080
Applications: IHC
Type: Rabbit IgG
Dilutions: IHC 1:1000-1:2000
Immunogen: Whole molecule 5HT
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Whole serum
Format B: lyophilized
Species Reactivity: Mouse, Rat
Entrez: 8879
UniProt: O95470
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$335.00Add to Cart

The histochemical antibody for serotonin is generated in a rabbit against serotonin coupled to bovine serum albumin with paraformaldehyde.

The antibody produces a 4+ indirect immunofluorescence staining at a 1/1,000 - 1/2,000 dilution and a 4+ biotin-streptavidin/HRP staining at a 1/20,000 - 1/40,000 dilution in rat hypothalamus, raphe nuclei and spinal cord. Staining is completely eliminated by pretreatment of the diluted antibody with 25 µg of serotonin/BSA.

Cross reactivity of Serotonin antisera was examined. With 5 µg, 10 µg and 25 µg amounts the following substances did not react with Serotonin antisera diluted 1/20,000 using the Bn-SA/HRP labeling method: 5-hydroxytryptophan, 5-hydroxyindole -3- acetic acid, and dopamine.

Images

5HT Serotonin staining of rat hypothalamus

5HT Serotonin staining of rat hypothalamus