The histochemical antibody for serotonin is generated in a rabbit against serotonin coupled to bovine serum albumin with paraformaldehyde.
The antibody produces a 4+ indirect immunofluorescence staining at a 1/1,000 - 1/2,000 dilution and a 4+ biotin-streptavidin/HRP staining at a 1/20,000 - 1/40,000 dilution in rat hypothalamus, raphe nuclei and spinal cord. Staining is completely eliminated by pretreatment of the diluted antibody with 25 µg of serotonin/BSA.
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