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Images
Immunohistochemical detection of BACE-1 in astrocytes in rat cortex. Rat brain was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to BACE-1 at 10 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200 dilution. Nuclei were counterstained with DAPI (blue).
Immunohistochemical detection of BACE-1 in astrocytes in rat cortex. Rat brain was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to BACE-1 at 10 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200 dilution. Nuclei were counterstained with DAPI (blue).
Immunohistochemical detection of BACE-1 in mouse cerebellum. Mouse brain was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to BACE-1 at 10 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200 dilution. Nuclei were counterstained with DAPI (blue)
Immunohistochemical detection of BACE-1 in mouse cerebellum. Mouse brain was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to BACE-1 at 10 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200 dilution. Nuclei were counterstained with DAPI (blue)
Immunohistochemical detection of BACE1 in reactive astrocytes in formalin fixed Alzheimer's human brain.12 μm paraffin-embedded tissue sections were incubated with rabbit polyclonal antibody to MMP1 at 8 μg/mL overnight at 4°C followed by HRP/AEC detection (red) and counterstaining with hematoxylin (blue).
Immunohistochemical detection of BACE1 in reactive astrocytes in formalin fixed Alzheimer's human brain.12 μm paraffin-embedded tissue sections were incubated with rabbit polyclonal antibody to MMP1 at 8 μg/mL overnight at 4°C followed by HRP/AEC detection (red) and counterstaining with hematoxylin (blue).