Product Details
Catalog Number: RA21012
Applications: ICC, WB, IHC, IF
Type: Rabbit IgG
Dilutions: Immunohistochemistry: 5-10 ug/ml Immunofluorescence: 2-15 ug/ml Western Blot: 1 ug/ml
Immunogen: Synthetic peptide
Storage: The product can be stored as supplied for up to 12 months at 2°C-4°C. After reconstitution, aliquot and store at -20°C for higher stability or at 4°C with an appropriate antibacterial agent. Avoid freeze thaw-cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: lyophilized
Species Reactivity: Human
Entrez: 836
UniProt: P42574
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ug$235.00Add to Cart

Caspase 3 is a heterotetramer of two heterodimers arranged in anti-parallel orientation. Caspase 3 activates caspases 6 and 7 and this sequential activation leads to apoptosis. CASP3 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Caspase 3 participates in cleavage of Amyloid beta 4A precursor protein which is implicated with neuronal death Alzheimer's disease. Caspase 3 in turn is processed by caspases 8, 9 and 10.

Images
Immunohistochemistry

Immunohistochemical detection of Caspase 3 in apoptosis induced Jurkat cells. Cells were fixed with 1% formaldehyde incubated with rabbit polyclonal antibody to N-terminal region of Caspase 3 at 6 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry

Immunohistochemical detection of Caspase 3 in apoptosis induced Jurkat cells. Cells were fixed with 1% formaldehyde incubated with rabbit polyclonal antibody to N-terminal region of Caspase 3 at 6 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200 dilution. Nuclei were counterstained with DAPI (blue).

Western Blot

Western blot analysis using RA21012

Western Blot

Western blot analysis using RA21012