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Immunohistochemistry

Immunohistochemical staining of Endomorphin 2 in rat ventral lateral periaqueductal grey matter (PAG). 4% paraformaldehyde fixed rat brain 10 μm thick cryostat sections were incubated with rabbit polyclonal antibody to Endomorphin 2 at 10 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. Tissue was counterstained with DAPI (blue color) to visualize cell nuclei.

Immunohistochemistry

Immunohistochemical staining of Endomorphin 2 in rat ventral lateral periaqueductal grey matter (PAG). 4% paraformaldehyde fixed rat brain 10 μm thick cryostat sections were incubated with rabbit polyclonal antibody to Endomorphin 2 at 10 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. Tissue was counterstained with DAPI (blue color) to visualize cell nuclei.

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