Product Details
Catalog Number: RA21019
Applications: ICC, WB, IHC, IF
Type: Rabbit IgG
Dilutions: Immunohistochemistry: 5-10 ug/ml Immunofluorescence: 5-15 ug/ml Western Blot: 1 ug/ml
Immunogen: Synthetic peptide
Storage: The product can be stored as supplied for up to 12 months at 2°C-4°C. After reconstitution, aliquot and store at -20°C for higher stability or at 4°C with an appropriate antibacterial agent. Avoid freeze thaw-cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat
Entrez: 3952
UniProt: P41159
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
50 ug$379.50Add to Cart

Leptin is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development.This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism.

Images
Immunohistochemistry

Immunohistochemical detection of Leptin in mouse hypothalamus. Mouse brain was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to Leptin at 15 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. Cell nuclei were counterstained with DAPI (blue).

Immunohistochemistry

Immunohistochemical detection of Leptin in mouse hypothalamus. Mouse brain was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to Leptin at 15 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. Cell nuclei were counterstained with DAPI (blue).

Western Blot

Western blot analysis using RA21019

Western Blot

Western blot analysis using RA21019