Images
Immunohistochemistry

Immunohistochemical detection of SMAD-2 in mouse skin. Tissue was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to SMAD-2 at 12 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. Nuclei of keratinocytes were counterstained with DAPI (blue).

Immunohistochemistry

Immunohistochemical detection of SMAD-2 in mouse skin. Tissue was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to SMAD-2 at 12 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. Nuclei of keratinocytes were counterstained with DAPI (blue).

Western Blot

Western blot analysis of RA21025

Western Blot

Western blot analysis of RA21025

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