Product Details
Catalog Number: RA22102
Applications: WB, IF
Type: Rabbit IgG
Dilutions: ICC 1:1000; IHC 1:1000; WB 1:10,000
Immunogen: recombinant alpha-internexin
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat
Entrez: 9118
UniProt: Q16352
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$303.00Add to Cart

alpha-internexin is a ~66 kDa Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M anbd NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.

Note: The serum can be diluted to 1:1,000 and higher for immunofluorescence staining and 1:10,000 and higher for western blotting. On western blots use a brain or spinal cord homogenate and look for a major band at SDS-PAGE apparent molecular weight of 66 to 68kDa, depending on the species. The human protein runs at ~68kDa, while rat, mouse and other species run at ~66kDa. Minor bands at ~150kDa are probably covalent dimers, and bands at ~50kDa represent a-internexin breakdown products.

Images

Mixed neuron-glial cultures stained with rabbit antibody to alpha-internexin (red) and chicken antibody to peripherin (green). The alpha internexin antibody stains numerous axonal and dendritic profiles in these cultures, while peripherin antibody binds to only a subset of neurons

Mixed neuron-glial cultures stained with rabbit antibody to alpha-internexin (red) and chicken antibody to peripherin (green). The alpha internexin antibody stains numerous axonal and dendritic profiles in these cultures, while peripherin antibody binds to only a subset of neurons

Western blot of whole rat spinal cord homogenate stained with RPCA-a-Int, at dilution of 1:20,000. A prominent band running at ~66kDa is apparent, as well as smaller lower bands which are apparently degradation products. A minor band at ~150kDa is also seen, apparently resulting from dimerization of alpha-internexin.

Western blot of whole rat spinal cord homogenate stained with RPCA-a-Int, at dilution of 1:20,000. A prominent band running at ~66kDa is apparent, as well as smaller lower bands which are apparently degradation products. A minor band at ~150kDa is also seen, apparently resulting from dimerization of alpha-internexin.