Product Details
Catalog Number: RA22105
Applications: ICC, WB, IHC
Type: Rabbit IgG
Dilutions: ICC 1:100; IHC 1:100; WB 1:1,000
Immunogen: Recombinant fusion protein containing the extreme C-terminal segment of rat NF-M
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Whole serum
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Chicken, Cat
Entrez: 4741
UniProt: P07197
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$303.00Add to Cart

Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.

Images

Section of rat cerebral cortex stained with NF-M (red), which reveals the perikarya of pyramidal neurons and dendrites and axons surrounding them. The green channel shows staining with a monoclonal antibody to the beta-adrendergic receptor kinase 1.

Section of rat cerebral cortex stained with NF-M (red), which reveals the perikarya of pyramidal neurons and dendrites and axons surrounding them. The green channel shows staining with a monoclonal antibody to the beta-adrendergic receptor kinase 1.

Western blot of whole rat cerebellum homogenate stained with NF-M, at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~145kDa corresponds to rodent NF-M. Human, cow and bovine NF-M run a little slower, at about 160kDa.

Western blot of whole rat cerebellum homogenate stained with NF-M, at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~145kDa corresponds to rodent NF-M. Human, cow and bovine NF-M run a little slower, at about 160kDa.