Product Details
Catalog Number: RA22108
Applications: ICC, WB, IF
Type: Rabbit IgG
Immunogen: Recombinant Coronin 1a expressed in and purified from E. coli.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Whole serum
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Cat
Entrez: 11151
UniProt: P31146
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$303.00Add to Cart

Human Coronin 1A is a 51,026 dalton protein (461 amino acids) expressed intrcellularly in macrophages and other cell types of the immune system.  In the CNS, coronin 1A is expressed exclusively by microglial cells.  Although its function is not entirely clear, there are reasons to believe that coronin serves as a linkage protein between the actin cytoskeleton and the plasma membrane, and perhaps the extracellular matrix.

The protein is found exclusively in hematopoetic lineage cells such as lymphocytes, macrophages and neutrophils. Coronin1a is therefore an excellent marker of cells of this lineage and can also be used to study the leading edges particularly of neutrophils. Since the only hematopoetic cells found within the central nervous system are microglia, this antibody is also an excellent marker of this important cell type*. Microglia are numerically fairly minor components of the nervous system, but microglial activation is seen in response to a wide variety of damage and disease states, including ALS, Alzheimer's disease and responses to brain tumors. Since coronin 1a is a constitutive component of microglia, the coronin 1a antibody can be used to study both quiescent and activated microglia.

Images

Immunocytochemistry of a mixed neuron/glial culture from newborn rat brain stained with Coronin 1a (green) at a dilution of 1:10,000 and chicken polyclonal Vimentin (1:5,000). Blue is nuclear DNA counter stain. Glial cells and fibroblasts stain with vimentin, while microglia alone stain strongly and specifically for Coronin 1a, which can therefore be used as a robust marker of this important cell type.

Immunocytochemistry of a mixed neuron/glial culture from newborn rat brain stained with Coronin 1a (green) at a dilution of 1:10,000 and chicken polyclonal Vimentin (1:5,000). Blue is nuclear DNA counter stain. Glial cells and fibroblasts stain with vimentin, while microglia alone stain strongly and specifically for Coronin 1a, which can therefore be used as a robust marker of this important cell type.

Western blot of HL60 Cell extract stained with Coronin 1a, at dilution of 1:10,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~57kDa corresponds to Coronin 1a. Numbers represent positions of molecular weight standards in kDa.

Western blot of HL60 Cell extract stained with Coronin 1a, at dilution of 1:10,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~57kDa corresponds to Coronin 1a. Numbers represent positions of molecular weight standards in kDa.