Product Details
Catalog Number: RA22116
Applications: ICC, WB, IHC
Type: Rabbit IgG
Dilutions: ICC 1:1000; IHC 1:1000; WB 1:10,000
Immunogen: Bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. The form of NF-H isolated is the heavily phosphorylated axonal variant, and the antibody generated recognizes predominantly axonal neurofilaments.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Whole serum
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Primate, Chicken
Entrez: 4744
UniProt: P12036
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$303.00Add to Cart

Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.

The form of NF-H isolated is the heavily phosphorylated axonal variant, and the antibody generated recognizes predominantly axonal neurofilaments.

Images

Section of rat spinal cord stained with NF-H, phosphylated (green) and monoclonal antibody to UCHL1 (red). The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large UCHL1 positive neurons.

Section of rat spinal cord stained with NF-H, phosphylated (green) and monoclonal antibody to UCHL1 (red). The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large UCHL1 positive neurons.

Lane labelled CBB shows Coomassie Brilliant Blue stained crude extract of rat spinal cord, with the prominent major neurofilament subunit indicated; In rodents, NF-H runs at 200kDa, NF-M at 145kDa and NF-L at 68kDa. A prominent band at an apparent SDS-PAGE molecular weight of 200kDa corresponds to phosphorylated form of NF-H. The lane marked Rab shows a similar blot probed with our rabbit antibody to NF-H.

Lane labelled CBB shows Coomassie Brilliant Blue stained crude extract of rat spinal cord, with the prominent major neurofilament subunit indicated; In rodents, NF-H runs at 200kDa, NF-M at 145kDa and NF-L at 68kDa. A prominent band at an apparent SDS-PAGE molecular weight of 200kDa corresponds to phosphorylated form of NF-H. The lane marked Rab shows a similar blot probed with our rabbit antibody to NF-H.