Vimentin is a protein which assembles to produce 10mm or intermediate filaments, which are major components of the cellular cytoskeleton. This protein is expressed in microglia, developing astrocytes, developing neurons, fibroblasts and endothelial cells in the developing nervous system. Levels of vimentin generally are reduced as development proceeds and in adult animals vimentin is mostly found in mesenchymal tissues. Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. The immunogen used to generate our antibody was recombinant human vimentin expressed in and purified from E. coli.
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