Product Details
Catalog Number: RA22125
Applications: ICC, WB, IHC, IF
Type: Rabbit IgG
Immunogen: Amino acids 315-630 of the human protein
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Whole serum
Format B: liquid
Species Reactivity: Human
Entrez: 10763
UniProt: P48681
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$325.00Add to Cart

The protein proved to be an unusual member of the intermediate filament family, containing an α-helical region homologous to that found in keratin and neurofilament subunits. The Human Genome Organization (HUGO) Gene Nomenclature Committee (HGNC) name for Nestin is NES.

Nestin is relatively poorly conserved in protein sequence across species boundaries, so that the mouse and human proteins have an overall identity of only 62%. As a result antibodies to the human protein often fail to recognize the rodent homologue and vice versa. Antibodies to nestin are widely used to identify neural stem cells. This antibody was raised in rabbit against recombinant human nestin from aa 315-630, which has only 55% identity to homologous mouse sequence. Nestin (RA22125) therefore recognizes nestin protein in human, but not in rodent. 

Images

SH-SY5Y neuroblastoma cells were stained with Nestin (RA221025) at 1:5,000 (red) and CH22108 against vimentin (green) at 1: 10,000. Vimentin is main partner of nestin forming heterdimers and further polymerizing to form intermediate filaments. Co-localization of vimentin and nestin in cells was shown in yellow. Blue is DAPI staining of DNA.

SH-SY5Y neuroblastoma cells were stained with Nestin (RA221025) at 1:5,000 (red) and CH22108 against vimentin (green) at 1: 10,000. Vimentin is main partner of nestin forming heterdimers and further polymerizing to form intermediate filaments. Co-localization of vimentin and nestin in cells was shown in yellow. Blue is DAPI staining of DNA.