Product Details
Catalog Number: RA22132
Applications: ICC, WB, IF
Type: Rabbit IgG
Dilutions: Western Blot: 1:2,000. Immunocytochemistry/Immunofluorescence: 1:1,000
Immunogen: Full length recombinant human DJ-1 expressed in and purified from E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Whole serum
Format B: liquid
Species Reactivity: Human, Mouse, Rat
Entrez: 11315
UniProt: Q99497
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$295.00Add to Cart

DJ1, also known as PARK7, is a soluble protein which belongs to the ThiJ/Pfp1/DJ1 superfamily. DJ1 was originally cloned as an oncogene that cooperatively transforms cells together with H-ras (1). Mutations in the DJ1 gene are associated with rare forms of autosomal recessive early-onset Parkinson’s disease (2). DJ-1 is a multifunctional protein that participates in transcriptional regulation, anti oxidative stress reaction, and chaperone, protease, and mitochondrial regulation. DJ1 may also function as an sensor for oxidative stress, and it protects neurons against oxidative stress and cell death (3-5). DJ-1 is expressed in almost all cells and tissues. DJ-1 is located in the cytoplasm, nucleus, and mitochondria in cells, and secreted DJ-1 has been observed in various cultured cells and tissues. Augmenting DJ1 activity might provide novel approaches to treating chronic neurodegenerative illnesses such as Parkinson’s disease and acute damage such as stroke (6). The HGNC name for this protein is PARK7. The antibody was generated against full length recombinant human DJ1 protein expressed in and purified from E. coli. This antibody binds to the human, rat and mouse DJ1 proteins. The rodent protein is about 91% identical to the human protein.

Images
DJ1/Park7 Immunofluorescence

Immunofluorescent analysis analysis of SH-SY5Y cells stained with rabbit pAb against DJ1/Park7, RA22132, dilution 1:1,000 in red, and costained with EnCor mouse mAb to actin, dilution 1:200 in green. The blue is Hoechst staining of nuclear DNA.

DJ1/Park7 Immunofluorescence

Immunofluorescent analysis analysis of SH-SY5Y cells stained with rabbit pAb against DJ1/Park7, RA22132, dilution 1:1,000 in red, and costained with EnCor mouse mAb to actin, dilution 1:200 in green. The blue is Hoechst staining of nuclear DNA.

DJ1/Park7 Western Blot

Western blot analysis analysis of different tissue and cell lysates using rabbit pAb against DJ1/Park7, RA22132, dilution 1:2,000 in green. [1] protein standard, [2] rat brain, [3] mouse brain, [4] rat embryonic neuron-glial cells, [5] NIH-3T3, [6] HEK293, and [7] HeLa cells.

DJ1/Park7 Western Blot

Western blot analysis analysis of different tissue and cell lysates using rabbit pAb against DJ1/Park7, RA22132, dilution 1:2,000 in green. [1] protein standard, [2] rat brain, [3] mouse brain, [4] rat embryonic neuron-glial cells, [5] NIH-3T3, [6] HEK293, and [7] HeLa cells.