Product Details
Catalog Number: RA22133
Applications: ICC, WB, IHC, IF
Type: Rabbit IgG
Dilutions: Western Blot: 1:1,000-5,000. Immunocytochemistry/Immunofluorescence: 1:2,000-5,000
Immunogen: Peptide identical to part of the C-terminal of human IBA1 coupled to KLH.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Whole serum
Format B: liquid
Species Reactivity: Human, Mouse, Rat
Entrez: 199
UniProt: P55008
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ug$295.00Add to Cart

IBA1, also known as AIF1, is a 17kDa member of the “EF” hand superfamily of Calcium binding proteins which is expressed in hematopoetic cells. Since the only hematopoetic cells normally found within the CNS are microglia, suitable IBA1 antibodies are widely used to identify microglial cells in sections and tissues. Microglia are the immunocompetent cells of the CNS and are extremely important in responses to CNS injury and disease.

Images
IBA1 High Magnification

High magnification stacked confocal image of rat cerebellar molecular layer at top and granular layer below, stained with RA22133, dilution 1:1,000, in green. Microglia are very small cells with fine processes spreading in three dimensions and so are best visualized in a confocal Z stack. Red shows the processes of Purkinje cells and the perikarya of granule cells revealed with an antibody to MAP2, 1:5,000.

IBA1 High Magnification

High magnification stacked confocal image of rat cerebellar molecular layer at top and granular layer below, stained with RA22133, dilution 1:1,000, in green. Microglia are very small cells with fine processes spreading in three dimensions and so are best visualized in a confocal Z stack. Red shows the processes of Purkinje cells and the perikarya of granule cells revealed with an antibody to MAP2, 1:5,000.

IBA1 Western Blot

Western blot analysis of different tissue lysates using rabbit pAb to IBA1, RPCA-IBA1, dilution 1:1,000 in green: [1] protein standard (red), [2] mouse brain, [3] rat brain, [4] mouse spleen, and [5] rat spleen.

IBA1 Western Blot

Western blot analysis of different tissue lysates using rabbit pAb to IBA1, RPCA-IBA1, dilution 1:1,000 in green: [1] protein standard (red), [2] mouse brain, [3] rat brain, [4] mouse spleen, and [5] rat spleen.