Product Details
Catalog Number: RA22134
Applications: WB, IHC, IF
Type: Rabbit IgG
Dilutions: Immunofluorescence: 1:2,000-5,000 Immunohistochemistry: 1:2,000-5,000 Western Blot: 1:1,000-5,000
Immunogen: Recombinant Green Fluorescent Protein purified from E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: liquid
Species Reactivity: Not Applicable
Entrez: 7011691
UniProt: P42212
Downloads: Datasheet (pdf)
Product Sizes
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100 ul of 1 mg/ml$303.00Add to Cart

GFP (green fluorescent protein) is 27kDa protein; which was original isolated from the jellyfish (Aequoria victoria). This antibody was made against a recombinant GFP, from the Aequoria species, referred to as AcGFP. This antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals.

Images
Immunofluorescent

Immunofluorescent analysis of transfected HEK293 cells with a GFP construct, in green, and stained with rabbit pAb to GFP, RA22134, dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The GFP antibody reveals GFP protein expressed only in transfected cells, and as a result these cells appear orange-yellow in color.

Immunofluorescent

Immunofluorescent analysis of transfected HEK293 cells with a GFP construct, in green, and stained with rabbit pAb to GFP, RA22134, dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The GFP antibody reveals GFP protein expressed only in transfected cells, and as a result these cells appear orange-yellow in color.

Western Blot

Western blot analysis of lysates of transfected HEK293 using rabbit pAb to GFP (in green), dilution 1:2,000. [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct, and [4] cells transfected with mCherry construct.

Western Blot

Western blot analysis of lysates of transfected HEK293 using rabbit pAb to GFP (in green), dilution 1:2,000. [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct, and [4] cells transfected with mCherry construct.