Product Details
Catalog Number: RA22138
Applications: ICC, WB, IF
Type: Rabbit IgG
Dilutions: Immunofluorescence: 1:5,000 Immunocytochemistry: 1:5,000 Western Blot: 1:10,000-15,000
Immunogen: C-terminal peptide of rat NF-L protein, GEEEDTKESEEEEKKEESAGEEQAAKKKD with an N-terminal Cys for coupling to KLH
Storage: Store at 4°C short term. Store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Whole serum
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine
Entrez: 4747
UniProt: P07196
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$325.00Add to Cart

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins may be included also. The major function of neurofilaments is likely to control the diameter of large axons. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70kDa with some variability across species. Antibodies to NF-L are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrig’s disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and others. Much interest has recently been focused on the detection of NF-L released from neurons into blood and CSF as a surrogate marker of primarily axonal loss in a variety of types of CNS injury and degeneration.

Images
Immunofluorescence

Immunofluorescent analysis of mouse cerebellum section stained with rabbit pAb to NF-L dilution 1:5,000 in red, and costained with chicken pAb to MBP dilution 1:5,000, in green.

Immunofluorescence

Immunofluorescent analysis of mouse cerebellum section stained with rabbit pAb to NF-L dilution 1:5,000 in red, and costained with chicken pAb to MBP dilution 1:5,000, in green.

Western Blot

Western blot analysis of different tissue lysates using rabbit pAb to NF-L, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.

Western Blot

Western blot analysis of different tissue lysates using rabbit pAb to NF-L, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.