SARS-CoV2 S-Protein ACE2 Binding Domain

Immunofluorescent analysis of HEK293 cells transfected with pCI-Neo-Mod vector (5) including DNA encoding the SARS-CoV2 S-protein ACE2 binding domain, amino acids 308-541 in the S-protein sequence. This region was used as the immunogen for development of rabbit antibody RA22140. The antibody was used at a dilution 1:3,000, shown in red. Cells costained with mAb to β-tubulin, dilution 1:5,000, in green. The blue is Hoechst staining of nuclear DNA.

Western blot analysis of HEK293 cell lysates using rabbit pAb to SARS-CoV2-bd protein, dilution 1:2,000 in green: [1] protein standard, [2] non-transfected cells, [3] cells transfected with pCI-Neo-Mod vector containing the SARS-Cov-bd cDNA, and [4] cells transfected with pCI-Neo-GFP vector containing the SARS-CoV-bd cDNA.