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Images
Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat embryos stained with rabbit pAb to αII-spectrin, RA22142, dilution 1:500 in green, and costained with mouse mAb to Ankyrin3 in red. The blue is Hoechst staining of nuclear DNA. The spectrin antibody stains submembranous cytoskeleton of neuronal cells. The Ankyrin3 antibody labels axonal initial segments of neurons.
Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat embryos stained with rabbit pAb to αII-spectrin, RA22142, dilution 1:500 in green, and costained with mouse mAb to Ankyrin3 in red. The blue is Hoechst staining of nuclear DNA. The spectrin antibody stains submembranous cytoskeleton of neuronal cells. The Ankyrin3 antibody labels axonal initial segments of neurons.
Western blot analysis of different tissue lysates using rabbit pAb to αII-spectrin, RA22142, dilution 1:2,000 in green: [1] protein standard (in red), [2] rat brain, [3] mouse spinal cord, and [4] cow cortex. The prominent band above 250kDa represents the intact αII-spectrin.
Western blot analysis of different tissue lysates using rabbit pAb to αII-spectrin, RA22142, dilution 1:2,000 in green: [1] protein standard (in red), [2] rat brain, [3] mouse spinal cord, and [4] cow cortex. The prominent band above 250kDa represents the intact αII-spectrin.