Product Details
Catalog Number: RA22142
Applications: ICC, WB, IHC, IF
Type: Rabbit IgG
Dilutions: Immunofluorescence: 1:500-1,000; Immunohistochemistry: 1:500-1,000; Western Blot: 1:2,000-5,000
Immunogen: Recombinant constructs spanning most of human alpha-II spectrin expressed in and purified from E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Bovine
Entrez: 6709
UniProt: Q13813
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

Spectrin family molecules are important high molecular weight components of the submembranous cytoskeleton of eukaryotic cells. These proteins were isolated originally from lysed red blood cell membrane preparations which were named “ghosts”, which gave rise to the name spectrin. They function as major components of the membraneous cytoskeleton, mediating interactions between integral membrane proteins, actin and many other cellular components. This antibody binds specifically to αII-spectrin, also known as non-erythroid spectrin or fodrin. In the CNS this protein is expressed only in neurons and so the antibody can be used to reveal the submembranous neuronal cytoskeleton in IF, ICC and IHC.

RA22142 was made against the recombinant human protein construct derived from the C-terminus of αII-spectrin comprising the C-terminal 14 spectrin repeats, specifically amino acids 676-2447. This antibody can be used to study αII-spectrin on western blots and to visualize the neuronal plasma membrane cytoskeleton in cells in culture and sectioned material.

Images
Immunofluorescence

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat embryos stained with rabbit pAb to αII-spectrin, RA22142, dilution 1:500 in green, and costained with mouse mAb to Ankyrin3 in red. The blue is Hoechst staining of nuclear DNA. The spectrin antibody stains submembranous cytoskeleton of neuronal cells. The Ankyrin3 antibody labels axonal initial segments of neurons.

Immunofluorescence

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat embryos stained with rabbit pAb to αII-spectrin, RA22142, dilution 1:500 in green, and costained with mouse mAb to Ankyrin3 in red. The blue is Hoechst staining of nuclear DNA. The spectrin antibody stains submembranous cytoskeleton of neuronal cells. The Ankyrin3 antibody labels axonal initial segments of neurons.

Western Blot

Western blot analysis of different tissue lysates using rabbit pAb to αII-spectrin, RA22142, dilution 1:2,000 in green: [1] protein standard (in red), [2] rat brain, [3] mouse spinal cord, and [4] cow cortex. The prominent band above 250kDa represents the intact αII-spectrin.

Western Blot

Western blot analysis of different tissue lysates using rabbit pAb to αII-spectrin, RA22142, dilution 1:2,000 in green: [1] protein standard (in red), [2] rat brain, [3] mouse spinal cord, and [4] cow cortex. The prominent band above 250kDa represents the intact αII-spectrin.