Product Details
Catalog Number: RA22143
Applications: ICC, WB, IF
Type: Rabbit IgG
Dilutions: Immunofluorescence: 1:2,000-5,000; Western Blot: 1:10,000-20,000
Immunogen: Full length human annexin A6 expressed in and purified from E. coli.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Dog, Equine
Entrez: 309
UniProt: P08133
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

The annexins are a large family of related proteins which share the property of binding to phophospholipid containing membranes in a Calcium dependent manner. Annexin A6 has a particularly surprising number of alternate names, 19 being listed on the Genecards ANXA6 site. Annexin A6 is normally localized in the cytosol but becomes membrane associated following cellular injury, and so is often seen in ““blebs”, transient herniations of the plasma membrane associated with apoptosis and more generally indicative of cell stress. Recent studies suggest that annexin A6 functions, in combination with annexin A4, in the repair of cell membranes following cellular injury.

RA22143 was made against full length recombinant human annexin A6 expressed in and purified from E. coli. The antibody recognizes annexin A6 in in human, rodents and many other mammals and can be used as a marker of blebs, indicative of cellular stress.

Images
Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with rabbit pAb to annexin A6, RA22143, dilution 1:2,000 in green, and costain with mouse mAb to β-tubulin in red. The blue is Hoechst staining of nuclear DNA. The annexin A6 antibody detects protein mainly expressed in spherical cellular membrane protrusions, known as blebs, while the β-tubulin antibody produces strong staining of cytoplasmic microtubules.

Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with rabbit pAb to annexin A6, RA22143, dilution 1:2,000 in green, and costain with mouse mAb to β-tubulin in red. The blue is Hoechst staining of nuclear DNA. The annexin A6 antibody detects protein mainly expressed in spherical cellular membrane protrusions, known as blebs, while the β-tubulin antibody produces strong staining of cytoplasmic microtubules.

Western Blot

Western blot analysis of different cell lines lysates probed with rabbit pAb to annexin A6, RA22143, dilution 1:10,000 in green: [1] protein standard (red), [2] mouse NIH-3T3, [3] rat C6, [4] human HeLa, [5] human HEK293, [6] dog A72, [7] equine NBL6 and [8] African green monkey COS1 cells. The strong band at about 68kDa corresponds to the annexin A6 protein.

Western Blot

Western blot analysis of different cell lines lysates probed with rabbit pAb to annexin A6, RA22143, dilution 1:10,000 in green: [1] protein standard (red), [2] mouse NIH-3T3, [3] rat C6, [4] human HeLa, [5] human HEK293, [6] dog A72, [7] equine NBL6 and [8] African green monkey COS1 cells. The strong band at about 68kDa corresponds to the annexin A6 protein.