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Immunofluorescent analysis of HeLa cells stained with rabbit pAb to annexin A6, RA22143, dilution 1:2,000 in green, and costain with mouse mAb to β-tubulin in red. The blue is Hoechst staining of nuclear DNA. The annexin A6 antibody detects protein mainly expressed in spherical cellular membrane protrusions, known as blebs, while the β-tubulin antibody produces strong staining of cytoplasmic microtubules.
Immunofluorescent analysis of HeLa cells stained with rabbit pAb to annexin A6, RA22143, dilution 1:2,000 in green, and costain with mouse mAb to β-tubulin in red. The blue is Hoechst staining of nuclear DNA. The annexin A6 antibody detects protein mainly expressed in spherical cellular membrane protrusions, known as blebs, while the β-tubulin antibody produces strong staining of cytoplasmic microtubules.
Western blot analysis of different cell lines lysates probed with rabbit pAb to annexin A6, RA22143, dilution 1:10,000 in green: [1] protein standard (red), [2] mouse NIH-3T3, [3] rat C6, [4] human HeLa, [5] human HEK293, [6] dog A72, [7] equine NBL6 and [8] African green monkey COS1 cells. The strong band at about 68kDa corresponds to the annexin A6 protein.
Western blot analysis of different cell lines lysates probed with rabbit pAb to annexin A6, RA22143, dilution 1:10,000 in green: [1] protein standard (red), [2] mouse NIH-3T3, [3] rat C6, [4] human HeLa, [5] human HEK293, [6] dog A72, [7] equine NBL6 and [8] African green monkey COS1 cells. The strong band at about 68kDa corresponds to the annexin A6 protein.