Product Details
Catalog Number: RA22146
Applications: ICC, WB, IHC, IF
Type: Rabbit IgG
Dilutions: Immunofluorescence: 1:2,000; Immunohistochemistry: 1:2,000; Western Blot: 1:5,000-10,000
Immunogen: Full length recombinant human protein expressed in and purified from E. coli.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat
Entrez: 2353
UniProt: P01100
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$299.00Add to Cart

The FOS gene and protein were originally identified as the transforming element in a viral oncogene. The transforming protein was named v-FOS, for viral FOS, and the normal cellular non-transforming proto-oncogene was called c-FOS, for cellular FOS The c-FOS protein is a normal gene acting as an on/off switch controlling the expression of many other genes. Since c-FOS expression is induced in neurons which are rapidly firing action potentials, appropriate c-Fos antibodies can be used to identify activated neurons in tissues.

RA22146 was made against recombinant full length human c-FOS expressed in and purified from E. coli. It can be used to identify activated cells in cell culture and in sections and to follow c-FOS expression in western blots of cell and tissue homogenates.

Images
Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with rabbit pAb to c-FOS, RA22146, dilution 1:2,000 in red, and mouse mAb to tubulin in green. The blue is DAPI staining of nuclear DNA.

Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with rabbit pAb to c-FOS, RA22146, dilution 1:2,000 in red, and mouse mAb to tubulin in green. The blue is DAPI staining of nuclear DNA.

Western Blot

Western Blot