Images
Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with rabbit pAb to fibrillarin, RA22147, dilution 1:5,000 in red, and costained with chicken pAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. RA22147 detects protein expressed in nucleoli of cells, while the vimentin antibody produces strong staining of cytoplasmic intermediate filaments.

Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with rabbit pAb to fibrillarin, RA22147, dilution 1:5,000 in red, and costained with chicken pAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. RA22147 detects protein expressed in nucleoli of cells, while the vimentin antibody produces strong staining of cytoplasmic intermediate filaments.

Western Blot

Western blot analysis of cell lysates using rabbit pAb to fibrillarin, RA22147, dilution 1:5,000, in green: [1] protein standard (red), [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, [6] C6, and [7] COS1 cells. The strong band at ~35kDa corresponds to the fibrillarin protein.

Western Blot

Western blot analysis of cell lysates using rabbit pAb to fibrillarin, RA22147, dilution 1:5,000, in green: [1] protein standard (red), [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, [6] C6, and [7] COS1 cells. The strong band at ~35kDa corresponds to the fibrillarin protein.

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