Product Details
Catalog Number: RA22147
Applications: ICC, WB, IHC, IP, IF
Type: Rabbit IgG
Dilutions: Immunofluorescence, Immunohistochemistry, Immunoprecipitation: 1:2,000-5,000 Western Blot: 1:2,000-5,000
Immunogen: Full length human fibrillarin expressed in and purified from E.Coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat
Entrez: 2091
UniProt: P22087
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ul$275.00Add to Cart

Fibrillarin is a highly conserved component of a nucleolar small ribonucleoprotein complex in mammals, involved in the processing of ribosomal RNA during ribosomal biogenesis. Fibrillarin was originally identified in humans since autoantibodies staining nucleoli were seen in some patients with the autoimmune disease scleroderma. Subsequently the protein fibrillarin was found to be the human homologue of Nop1p, a Saccharomyces cerevisiae nucleolar protein. Autoantibodies to fibrillarin are also seen in patients with the autoimmune disease systemic sclerosis.

RA22147 was made against recombinant human fibrillarin expressed in and purified from E. coli.

Images
Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with rabbit pAb to fibrillarin, RA22147, dilution 1:5,000 in red, and costained with chicken pAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. RA22147 detects protein expressed in nucleoli of cells, while the vimentin antibody produces strong staining of cytoplasmic intermediate filaments.

Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with rabbit pAb to fibrillarin, RA22147, dilution 1:5,000 in red, and costained with chicken pAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. RA22147 detects protein expressed in nucleoli of cells, while the vimentin antibody produces strong staining of cytoplasmic intermediate filaments.

Western Blot

Western blot analysis of cell lysates using rabbit pAb to fibrillarin, RA22147, dilution 1:5,000, in green: [1] protein standard (red), [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, [6] C6, and [7] COS1 cells. The strong band at ~35kDa corresponds to the fibrillarin protein.

Western Blot

Western blot analysis of cell lysates using rabbit pAb to fibrillarin, RA22147, dilution 1:5,000, in green: [1] protein standard (red), [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, [6] C6, and [7] COS1 cells. The strong band at ~35kDa corresponds to the fibrillarin protein.