Product Details
Catalog Number: RA22148
Applications: ICC, WB, IHC, IF
Type: Rabbit IgG
Dilutions: Immunofluorescence: 1:2,000; Immunohistochemistry: 1:2,000; Western Blot: 1:30,000
Immunogen: Full length recombinant human GAPDH expressed in and purified from E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Chicken, Bovine, Porcine, Equine
Entrez: 2597
UniProt: P00355
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate to produce glyceraldehyde 1-3-bisphosphate. Because GAPDH protein is expressed in large amounts and is required at all times for an important “house keeping” function, levels of GAPDH mRNA are often measured and used as standards in studies of the expression of other mRNAs. Similarly specific antibodies to GAPDH are used to measure GAPDH expression as a protein standard in western blotting experiments. This antibody can be used as a loading control for such experiments, allowing comparison between the expression level of this protein and others in a cell or tissue.

The immunogen used to raise RA22148 was extensively purified pig GAPDH. The antibody has been widely used as a western blotting standard by many labs for many years and is known to detect GAPDH in a variety of mammalian species including human, rat and mouse. It produces a single clean band on western blots of typical cell and tissue extracts.

Images
Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with rabbit pAb to GAPDH, RA22148, dilution 1:2,000 in red. Blue is Hoechst staining of nuclear DNA. RA22148 produces diffuse cytoplasmic staining of cells.

Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with rabbit pAb to GAPDH, RA22148, dilution 1:2,000 in red. Blue is Hoechst staining of nuclear DNA. RA22148 produces diffuse cytoplasmic staining of cells.

Western Blot

Western blot analysis of different cell cytosolic or nuclear enriched fractions, using rabbit pAb to GAPDH, RA22148, dilution 1:20,000, in red: [1] protein standard, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. Strong band at 37kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions.

Western Blot

Western blot analysis of different cell cytosolic or nuclear enriched fractions, using rabbit pAb to GAPDH, RA22148, dilution 1:20,000, in red: [1] protein standard, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. Strong band at 37kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions.