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Immunofluorescent analysis of HeLa cells stained with rabbit pAb to GAPDH, RA22148, dilution 1:2,000 in red. Blue is Hoechst staining of nuclear DNA. RA22148 produces diffuse cytoplasmic staining of cells.
Immunofluorescent analysis of HeLa cells stained with rabbit pAb to GAPDH, RA22148, dilution 1:2,000 in red. Blue is Hoechst staining of nuclear DNA. RA22148 produces diffuse cytoplasmic staining of cells.
Western blot analysis of different cell cytosolic or nuclear enriched fractions, using rabbit pAb to GAPDH, RA22148, dilution 1:20,000, in red: [1] protein standard, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. Strong band at 37kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions.
Western blot analysis of different cell cytosolic or nuclear enriched fractions, using rabbit pAb to GAPDH, RA22148, dilution 1:20,000, in red: [1] protein standard, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. Strong band at 37kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions.