Product Details
Catalog Number: RA22149
Applications: ICC, WB, IHC, IF
Type: Rabbit IgG
Dilutions: Immunofluorescence: 1:5,000; Immunohistochemistry: 1:5,000; Western Blot: 1:5,000-10,000
Immunogen: Recombinant full length human HSP60 expressed in and purified from E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Primate, Bovine, Dog, Porcine, Equine
Entrez: 3329
UniProt: P10809
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

The heat shock proteins were discovered, as the name suggests, since they are heavily upregulated when cells are stressed by temperatures above the normal physiological range. They are expressed in unstressed cells also and have a normal function as chaperones, helping other proteins to fold correctly. The need for chaperones is much greater if a cell or tissue is stressed by heat, and so these proteins become heavily up regulated.. In addition to its role as a heat shock protein, HSP60 plays an important role in the transport and maintenance of mitochondrial proteins as well as the transmission and replication of mitochondrial DNA. HSP60 has also been implicated in the initiation and/or progression of some subtypes of cardiovascular disease (CVD).

Images
Immunofluorescence

Confocal immunofluorescent analysis of HeLa cells stained with rabbit pAb to HSP60, RA22149, dilution 1:1,000, in red, and costained with chicken pAb to vimentin in green. The blue is DAPI staining of nuclear DNA. The HSP60 antibody gives strong and specific staining of mitochondria while the vimentin antibody reveals cytoplasmic intermediate filaments.

Immunofluorescence

Confocal immunofluorescent analysis of HeLa cells stained with rabbit pAb to HSP60, RA22149, dilution 1:1,000, in red, and costained with chicken pAb to vimentin in green. The blue is DAPI staining of nuclear DNA. The HSP60 antibody gives strong and specific staining of mitochondria while the vimentin antibody reveals cytoplasmic intermediate filaments.

Western Blot

Western blot analysis of different tissue or cell lysates using rabbit pAb to HSP-60, RA22149, dilution 1:5,000 in red. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong 60kDa band present in all preparations corresponds to HSP60 protein.

Western Blot

Western blot analysis of different tissue or cell lysates using rabbit pAb to HSP-60, RA22149, dilution 1:5,000 in red. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong 60kDa band present in all preparations corresponds to HSP60 protein.