Images
Immunofluorescence

Confocal immunofluorescent analysis of HeLa cells stained with rabbit pAb to HSP60, RA22149, dilution 1:1,000, in red, and costained with chicken pAb to vimentin in green. The blue is DAPI staining of nuclear DNA. The HSP60 antibody gives strong and specific staining of mitochondria while the vimentin antibody reveals cytoplasmic intermediate filaments.

Immunofluorescence

Confocal immunofluorescent analysis of HeLa cells stained with rabbit pAb to HSP60, RA22149, dilution 1:1,000, in red, and costained with chicken pAb to vimentin in green. The blue is DAPI staining of nuclear DNA. The HSP60 antibody gives strong and specific staining of mitochondria while the vimentin antibody reveals cytoplasmic intermediate filaments.

Western Blot

Western blot analysis of different tissue or cell lysates using rabbit pAb to HSP-60, RA22149, dilution 1:5,000 in red. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong 60kDa band present in all preparations corresponds to HSP60 protein.

Western Blot

Western blot analysis of different tissue or cell lysates using rabbit pAb to HSP-60, RA22149, dilution 1:5,000 in red. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong 60kDa band present in all preparations corresponds to HSP60 protein.

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