Ki67

Immunofluorescent analysis of HeLa cells stained with rabbit pAb to Ki67, RA22150, dilution 1:5,000 in red, and mouse monoclonal antibody to fibrillarin in green. The blue is DAPI staining of nuclear DNA. The Ki67 protein accumulates in and around the nucleoli of interphase cells such as those on the right, and the nucleoli are revealed by the fibrillarin antibody. In contrast, cells in the quiescent G0 state such as those on the left are Ki67 negative but fibrillarin positive.

Western blot analysis of equal amounts of cell lysates using rabbit pAb to Ki67, RA22150, dilution 1:10,000, (green): [1] protein standard (red), [2] rapidly growing HeLa cell cultures, [3] rapidly growing HEK293 cell cultures. Strong double bands larger than the 250kDa standard correspond to full length 345kDa and 395kDa Ki67 isoforms, while smaller proteolytic fragments of these isoforms are also invariably detected on the blot.